Purification and Characterization of a Novel Factor of Crotoxin Inter-CRO (V-1), a New Phospholipase A2 Isoform from Crotalus durissus collilineatus Snake Venom Using an In Vitro Neuromuscular Preparation

The fractionation of Crotalus durissus collilineatus whole venom through an HPLC chromatographic method enabled the purification a new V-1 neurotoxin. Inter-CRO (V-1) presents similarity in its primary structure to crotoxin B (CB), suggesting another isoform this toxin. aim study was compare complex (CA/CB) and CB elucidate aspects related functionality. homogeneity purified protein confirmed with molecular mass 1425.45 Da, further verified by spectrometry. sequence showed high other viperid snake PLA2 proteins. results report that is uncharacterized novel toxin different biological activities from CB. maintained catalytic activity but presented neurotoxic as observed 2.5-fold increase twitch tension record compared control values on isolated muscle cells.